Mouse Monoclonal Antibody to Beta Tubulin

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简述：This gene encodes a member of the beta tubulin protein FAmily. Beta tubulins are one of two core protein FAmilies (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is specifically expressed in platelets and megakaryocytes and may be involved in proplatelet production and platelet release. A mutations in this gene is associated with autosomal dominant macrothrombocytopenia. Two pseudogenes of this gene are found on chromosome Y.
应用：WB,IHC,IF,FCM
种属反应性：Human, Mouse, Monkey

Immunogen	Purified recombinant fragment of human TUBB1 (AA: 1-444) expressed in E. Coli.
Gene ID	203068
Swiss Prot	P07437
Synonyms	M40; TUBB1; TUBB5; CDCBM6; CSCSC1; OK/SW-cl.56
Tested applications	WB,IHC,IF,FCM
Calculated MW	50.3kDa
Source	Mouse
Clonality	Monoclonal Antibody
Isotype	Mouse IgG1
Storage/Stability	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Formulation	Purified antibody in PBS with 0.05% sodium azide
WB	1/1000 - 1/2000
IHC	1/200 - 1/1000
ICC	1/200 - 1/1000
FCM	1/200 - 1/400
ELISA	1/10000

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using TUBB1 mouse mAb against K562 (1), HepG2 (2), A431 (3), Jurkat (4), Hela (5), NIH/3T3 (6), and COS-7 (7) cell lysate.
Immunofluorescence analysis of Hela cells using TUBB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of Hela cells using TUBB1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using TUBB1 mouse mAb with DAB staining.