Mouse Monoclonal Antibody to LMNB1

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简述：This gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants.
应用：WB,IHC,IF,FCM
种属反应性：Human,Mouse,Rat

Immunogen	Purified recombinant fragment of human LMNB1 (AA: 413-583) expressed in HEK293-6e cells supernatant.
Gene ID	4001
Swiss Prot	P20700
Synonyms	LMN；ADLD；LMN2; LMNB；MCPH26
Calculated MW	66.4kDa
Source	Mouse
Clonality	Monoclonal Antibody
Isotype	Mouse IgG1
Storage/Stability	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Formulation	Purified antibody in PBS with 0.05% sodium azide
WB	1/500 - 1/2000
IHC	1/200 - 1/1000
ICC	1/200 - 1/1000
FCM	1/200 - 1/400
ELISA	1/10000

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using LMNB1 mouse mAb against Hela (1), HEK2933 (2), K562 (3), SH-SY5Y (4), and PC-3 (5) cell lysate.
Immunofluorescence analysis of Hela cells using LMNB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of Hela cells using LMNB1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human colon tissues using LMNB1 mouse mAb with DAB staining.