Mouse Monoclonal Antibody to HSP60

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简述：This gene encodes a member of the chaperonin FAmily. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related FAmily member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. (provided by RefSeq)
应用：WB,IHC,IF,FCM
种属反应性：Human,Mouse,Rat,Monkey

Immunogen	Purified recombinant fragment of human HSP60 expressed in E. Coli.
Gene ID	3329
Swiss Prot	P10809
Synonyms	HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60; HSPD1
Observed MW	61kDa
Source	Mouse
Clonality	Monoclonal Antibody
Isotype	Mouse IgG1
Storage/Stability	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Formulation	Ascitic fluid containing 0.03% sodium azide.
WB	1/500 - 1/2000
IHC	1/200 - 1/1000
ICC	1/200 - 1/1000
FCM	1/200 - 1/400
ELISA	1/10000

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)
Western blot analysis using HSP60 mouse mAb against T47D (1), Hela (2), HepG2 (3), A549 (4), Jurkat (5), HEK293 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate.
Immunofluorescence analysis of 3T3-L1 cells using HSP60 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric analysis of Hela cells using HSP60 mouse mAb (green) and negative control (purple).
Immunohistochemical analysis of paraffin-embedded human lung cancer tissues (left) and kidney cancer tissues (right) using HSP60 mouse mAb with DAB staining.