Mouse Monoclonal Antibody to beta-Actin

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简述：Beta-actin (PS1TP5-binding protein 1), also known as ACTB, PS1TP5BP1. Entrez Protein NP_001092. It is one of six different actin proteins. Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton.Actins are highly conserved proteins that are involved in various types of cell motility, structure, and integrity. Actin is ubiquitously expressed in all eukaryotic cells. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
应用：WB,IP,IF,FCM
种属反应性：Human,Mouse,Hamster,Rat,Monkey,Dog

Immunogen	Synthetic peptide corresponding to amino-terminal residues of human beta-Actin, conjugated to KLH.
Gene ID	60
Swiss Prot	P60709
Synonyms	PS1TP5BP1; ACTB
Tested applications	WB,IP,IF,FCM
Observed MW	42kDa
Source	Mouse
Clonality	Monoclonal Antibody
Isotype	Mouse IgG2b
Storage/Stability	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Formulation	Purified antibody in PBS with 0.05% sodium azide.
WB	1/10000 - 1/40000
IP	1/100-1/200
ICC	1/50 - 1/500
FCM	1/200 - 1/400
ELISA	1/10000

Western blot analysis using beta-Actin mouse mAb against NIH/3T3 (1), Jurkat (2), Hela (3), CHO (4), PC12 (5), HEK293 (6), COS (7), A549 (8) and MCF-7 (9) cell lysate.
Western blot analysis using beta-Actin mouse mAb against Rat serum(1),Rabbit serum(2),PC-12(3),Raw264.7(4),NIH/3T3(5),NRK(6),C2C12(7),C6(8),L1210(9),F9(10),CHO3D10(11),Hela(12),Jurkat(13) cell lysate.
Immunoprecipitation using beta-Actin mouse mAb(dilution: 1/250) against HL-60,C2C12,NRK,RSC,Hela,Cos7 cell lysate.
Confocal Immunofluorescence analysis of SKBR-3 (left) and A549 (right) cells using beta Actin mouse mAb (red, the secondary Ab is Cy3-Goat anti mouse IgG). Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric analysis of MCF-7 cells using beta Actin mouse mAb (right) and negative control (left).