Mouse Monoclonal Antibody to COX4I1

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简述：Cytochrome c oxidase (COX) functions as the terminal oxidase of the respiratory chain that uses cytochrome c as an electron donor to drive a proton gradient across the inner mitochondrial membrane. The mammalian COX apoenzyme is a heteromer consisting of three mitochondrial encoded catalytic subunits and several nuclear gene encoded structural subunits. COX contains two iron-coordination sites and two copper-coordination sites. Cytochrome c oxidase IV (COX4) is a nuclear-encoded subunit of COX that may play a role in regulating COX activity. COX4 is expressed ubiquitously in adult human tissue with the strongest levels of expression in the pancreas and moderate expression levels in heart, skeletal muscle and placenta.
应用：WB,IF,FCM
种属反应性：Human,Mouse,Rat,Monkey

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Immunogen	Purified recombinant fragment of human COX4I1 expressed in E. Coli.
Gene ID	1327
Swiss Prot	P13073
Synonyms	COX4; COXIV; COX4-1; MGC72016; COX4I1
Observed MW	19kDa
Source	Mouse
Clonality	Monoclonal Antibody
Isotype	Mouse IgG1
Storage/Stability	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Formulation	Purified antibody in PBS with 0.05% sodium azide.
WB	1/500 - 1/2000
ICC	1/200 - 1/1000
FCM	1/200 - 1/400
ELISA	1/10000

Western blot analysis using COX4I1 mouse mAb against HEK293 (1), A549 (2) and PC12 (3) cell lysate.
Immunofluorescence analysis of PANC-1 cells using COX4I1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric analysis of K562 cells using COX4I1 mouse mAb (blue) and negative control (red).

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