Mouse Monoclonal Antibody to TUBB3

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简述：Tubulin, beta 3, also known as TUBB3. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non exchangeable site on the alpha-chain. Tubulin is a highly conserved protein with a molecular weight of ~50 kD. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton.The two main tubulin isoforms, a- and
应用：WB,IF,FCM
种属反应性：Human,Mouse,Rat

Immunogen	Purified recombinant fragment of human TUBB3 expressed in E. Coli.
Gene ID	10381
Swiss Prot	Q13509
Synonyms	tubulin, beta 3; MC1R; TUBB4
Tested applications	WB,IF,FCM
Calculated MW	50~55kDa
Source	Mouse
Clonality	Monoclonal Antibody
Isotype	Mouse IgG1
Storage/Stability	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Formulation	Purified antibody in PBS with 0.05% sodium azide
WB	1/500-1/2000
ICC	1/200 - 1/1000
FCM	1/200 - 1/400
ELISA	1/10000

Western blot analysis using TUBB3 mouse mAb against HepG2 (1), A549 (2) and Hela (3) cell lysate.
Immunofluorescence analysis of PANC-1 cells using TUBB3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric analysis of A549 cells using TUBB3 mouse mAb (green) and negative control (purple).