Mouse Monoclonal Antibody to TUBB3

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简述：This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants.
应用：WB,IHC,FCM
种属反应性：Human, Mouse

Immunogen	436-450:cMYEDDEEESEAQGPK
Gene ID	10381
Swiss Prot	Q13509
Synonyms	CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, beta-4
Observed MW	50kDa
Source	Mouse
Clonality	Monoclonal Antibody
Isotype	Mouse IgG1
Storage/Stability	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Formulation	Purified antibody in PBS with 0.05% sodium azide
WB	1/500 - 1/2000
IHC	1/200 - 1/1000
FCM	1/200 - 1/400
ELISA	1/10000

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using TUBB3 mouse mAb against NIH/3T3 (1), Hela (2), and A549 (3) cell lysate.
Flow cytometric analysis of Hela cells using TUBB3 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using TUBB3 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human cerebellum tissues using TUBB3 mouse mAb with DAB staining.