Mouse Monoclonal Antibody to His Tag

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简述：The 6 X histidine motif is used as a tag on many recombinant proteins to FAcilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells.
应用：WB,IP,IHC,IF,FCM
种属反应性：Tag

Immunogen	Synthesized peptide of His Tag (AA: HHHHHHHH-KLH).
Synonyms	6 His tag; HHHHHH tag
Source	Mouse
Clonality	Monoclonal Antibody
Isotype	Mouse IgG1
Storage/Stability	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Formulation	Purified antibody in PBS with 0.05% sodium azide
WB	1/500-1/1000
IP	1/20-1/200
IHC	1/100 - 1/1000
ICC	1/100 - 1/1000
FCM	1/200 - 1/400
ELISA	1/10000

Red: Control Antigen (100ng) Purple: Antigen (10ng), Green: Antigen (50ng) Blue: Antigen (100ng),
Western blot analysis using His tag mAb against recombinant protein. lane 1 :(100 ng); lane 2 :(50 ng); lane 3 :(25 ng); lane 4 :(10 ng); lane 5 :(2.5 ng); (Expected MW is 50 kDa).