Mouse Monoclonal Antibody to GFP Tag

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简述：GFP (Green fluorescence protein) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors. GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.
应用：WB,IP,IF,IHC,FCM
种属反应性：Tag

Immunogen	Purified recombinant fragment of GFP expressed in E. Coli.
Swiss Prot	P42212
Tested applications	WB,IP,IF,IHC,FCM
Source	Mouse
Clonality	Monoclonal Antibody
Isotype	Mouse IgG2a
Storage/Stability	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Formulation	Purified antibody in PBS with 0.05% sodium azide
WB	1/500-1/1000
IP	1/20-1/50
IHC	1/200 - 1/1000
FCM	1/200 - 1/400
ELISA	1/10000
IF/ICC	1/100-1/200
产品分子量	27kDa

Flow cytometric analysis of HCC827 cells, untransfected (blue) or transfected with GFP (green), using GFP mouse mAb .
Immunocytochemistry analysis of HCC827 cells, untransfected (left) or transfected with GFP (right) using GFP mouse mAb .