Mouse Monoclonal Antibody to GST Tag

您当前的位置：

简述：Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. Expression vectors with Glutathione S-transferase (GST) tag have beed developed widely for both prokaryotic and eukaryotic systems, because GST can provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein encoded by inserted cDNA sequences. Antibodies of anti-GST tag are useful for checking protein expression using immunoblotting, immunoprecipitation and immunostaining techniques, as well as for immunoaffinity purification of protein expressed in vitro.
应用：WB,IP,IF,IHC,FCM
种属反应性： Tag

Immunogen	Purified fusion protein with GST protein tag expressed in E. Coli.
Clonality	Monoclonal Antibody
Isotype	Mouse IgG1
Storage/Stability	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Formulation	Purified antibody in PBS with 0.05% sodium azide
WB	1/500 - 1/2000
IP	1/10-1/20
IHC	1/100 - 1/1000
ICC	1/100 - 1/1000
FCM	1/200 - 1/400
ELISA	1/10000

Western blot analysis using GST mouse mAb against various fusion protein with GST tag.