Rabbit Monoclonal Antibody to Glucocorticoid Receptor

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简述：This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities.
应用：WB,IHC,IF,IP,ELISA
种属反应性：Human,Mouse,Rat,

Gene ID	2908
Swiss Prot	P04150
Synonyms	GCCR;GCR;GCR_HUMAN;GCRST;glucocorticoid nuclear receptor variant 1;Glucocorticoid receptor;GR;GRL;Grl1;nr3c1;Nuclear receptor subfamily 3 group C member 1;nuclear receptor subfamily 3, group C, member 1;glucocorticoid receptor;
Tested applications	WB,IHC,IF,IP,ELISA
Observed MW	85kD
Source	Rabbit
Clonality	Monoclonal Antibody
Isotype	Rabbit IgG,Kappa
Conjugation	Unmodified
Storage/Stability	-15°C to -25°C/1 year(Do not lower than -25°C)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
WB	1/1000-1/5000
IP	1/50-1/200
IHC	1/1000-1/4000
ICC	1/200-1/1000
ELISA	1/5000-1/20000

Western blot analysis of lysates from U-251 MG cells, using Glucocorticoid Receptor Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody was used to detect the antibody.
Immunohistochemical analysis of paraffin-embedded Human liver tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
Immunohistochemical analysis of paraffin-embedded Rat liver tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).