Mouse Monoclonal Antibody to NF-κB p65

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简述：NF-kappa-B is a pleiotropic transcription FActor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The heterodimeric RELA-NFKB1 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity.
应用：WB,IHC
种属反应性：Human,Mouse,Rat,Rabbit,Monkey

Immunogen	Purified recombinant fragment of human NF-κB p65 expressed in E. Coli.
Gene ID	5970
Swiss Prot	Q04206
Synonyms	NFkappaB p65; p65; NFKB3; RELA
Observed MW	65kDa
Source	Mouse
Clonality	Monoclonal Antibody
Isotype	Mouse IgG1
Storage/Stability	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Formulation	Purified antibody in PBS with 0.05% sodium azide.
WB	1/500 - 1/2000
IHC	1/100 - 1/500
ELISA	1/10000

Western blot analysis using NF-κB p65 mouse mAb against Jurkat (1), K562 (2) and NIH/3T3 (3) cell lysate.
Western blot analysis using NF-κB p65 mouse mAb against RSC-96(1),KO-SF(2),NIH/3T3(3),NRK(4),C2C12(5),C6(6),81505(7) cell lysate.
Immunohistochemical analysis of paraffin-embedded Mouse kidney(A) Mouse liver (B) using NF-κB p65 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Rat spleen(A) Rat liver (B) using NF-κB p65 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Rabbit spleen(A) Rabbit Rabbit liver(B) using NF-κB p65 mouse mAb with DAB staining.